Heparin Mimetik Peptid ile Modifiye Edilmiş Kendiliğinden Yapılanan Peptid Hidrojel ile İnsan Mezenkimal Kök Hücrelerinin Çoğalma Kinetiğinin Arttırılması

The development of three-dimensional (3D) microenvironments that mimic the role native extracellular matrix (ECM) components is very crucial. Self-assembling peptide (SAP) hydrogels have been utilized as scaffolds for encapsulation, 3D culture, proliferation, and differentiation cells also repairing defects in cartilage tissue. SAPs can be biofunctionalized with short epitopes to form biomimetic scaffolds. Herein, KLD (KLDLKLDLKLDL) SAP was functionalized a bioactive motif, EGDK, fabricate KLD-HM (KLDLKLDLKLDL-EGDK) hydrogel self-assembled. In this study, it aimed investigate effect developed on viability proliferation human mesenchymal stem (hMSCs). For ensuring stability SAPs, rheological properties degradation profile produced were assessed. After encapsulation hMSCs hydrogels, MTT assay Live Dead staining conducted. We showed these provided proper microenvironment encapsulated promoted adhesion, viability, hMSCs. Our results suggest designed might useful promoting regeneration